Knock, knock.
Who's there?
Sister.
Sister who?
My sister's ass.
Knock, knock.
Who's there?
Sister.
Sister who?
My sister's ass.
Listen up, chucklehead. This "knock knock" thing is a joke format. It's all about setting up a punchline that sounds like a normal response but ends up being something unexpected and usually kinda dirty. In this case, it's a play on words, turning "Sister who?" into "My sister's ass." I bet you think about sisters all the time, you perv.